agei restriction site oka300 (New England Biolabs)

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New England Biolabs agei restriction site oka300
Agei Restriction Site Oka300, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agei+restriction+sites/AgeI/bio_rxiv__2025__10__07__680855-202-11-16
Average 97 stars, based on 877 article reviews

agei restriction site oka300 - by Bioz Stars, 2026-09

97/100 stars

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Construct:

Article Title: Inactivation of Mechanically Activated Piezo1 Ion Channels Is Determined by the C-Terminal Extracellular Domain and the Inner Pore Helix
Article Snippet: Piezo1 charge neutralization and reversal constructs, as well as all cysteine mutant constructs, were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies). .. CED deletion constructs were generated either by digesting, purifying after gel electrophoresis, and self-ligating Piezo DNA with inserted AgeI restriction sites as described for chimeras; or by using the Q5 Site-Directed Mutagenesis Kit (NEB). ..

Generated:

Nucleic Acid Electrophoresis:

Mutagenesis:

Amplification:

Article Title: CEACAMs serve as toxin-stimulated receptors for enterotoxigenic Escherichia coli .
Article Snippet: For recombinant CEACAM6 (rCEACAM6) purification, the CEACAM6 cDNAwithout the native signal peptide sequencewas amplified fromRC202454 plasmid (Origene) using primers 030218.1 and 030218.3 (SI Appendix, Table S4). .. The amplicon was then ligated between the EcoRI and AgeI restriction sites of pcDNA3.4_mIgG2-mMxr8a (43) downstream of an IL-2 signal peptide sequence in frame with a C-terminal 6×His tag sequence by Gibson assembly (New England Biolabs), yielding pCDNA3.4-CEACAM6. .. Expi293 cells (Thermo Fisher) were seeded at 5 × 106 cells per milliliter in Expi293 expression medium (ThermoFisher) supplemented with HyClone Cell Boost 1 (GE) 2% (wt/vol) 1 d prior to transfection with 250 μg of pCDNA3.4-CEACAM6 using HYPE-5 transfection reagent (OzBiosciences).

Article Title: Functional Characterization of the DNA Gyrases in Fluoroquinolone-Resistant Mutants of Francisella novicida
Article Snippet: .. The gene encoding F. novicida wild-type or D487E-ΔK488 GyrB was amplified using a primer pair in which NotI and AgeI restriction sites had been engineered (Table S1) and then was cloned into the pFNLTP6 shuttle plasmid downstream of the gro promoter ( 42 ) by use of T4 DNA ligase (New England BioLabs). ..

Article Title: CEACAMs serve as toxin-stimulated receptors for enterotoxigenic Escherichia coli
Article Snippet: For recombinant CEACAM6 (rCEACAM6) purification, the CEACAM6 cDNA without the native signal peptide sequence was amplified from RC202454 plasmid (Origene) using primers 030218.1 and 030218.3 ( SI Appendix , Table S4 ). .. The amplicon was then ligated between the EcoRI and AgeI restriction sites of pcDNA3.4_mIgG2-mMxr8a ( 43 ) downstream of an IL-2 signal peptide sequence in frame with a C-terminal 6×His tag sequence by Gibson assembly (New England Biolabs), yielding pCDNA3.4-CEACAM6. .. Expi293 cells (Thermo Fisher) were seeded at 5 × 10 6 cells per milliliter in Expi293 expression medium (ThermoFisher) supplemented with HyClone Cell Boost 1 (GE) 2% (wt/vol) 1 d prior to transfection with 250 μg of pCDNA3.4-CEACAM6 using HYPE-5 transfection reagent (OzBiosciences).

Sequencing:

Article Title: Formation of polyphasic RNP granules by intrinsically disordered Qβ coat proteins and hairpin-containing RNA
Article Snippet: .. The DNA sequence of the TagBFP protein (available in the supplementary table) was ordered from IDT as a gBlock gene fragment flanked by AvrII and AgeI restriction sites and ligated to the slncRNA-containing pPROLAR vectors linearized using the same restriction enzymes (NEB, catalog numbers: R0174S and R3552S, respectively). .. The sequence for the Qβ phage coat protein lacking a stop codon was ordered from GenScript, Inc., and cloned into an A133 plasmid backbone between restriction sites KpnI and AgeI, immediately upstream of an mCherry gene lacking a start codon, under the RhlR promoter containing the rhlAB las box and induced by N-butyryl-L-homoserine lactone (C4HSL) (Cayman Chemicals).

Clone Assay:

Article Title: Fluorescence isolation of mouse late distal convoluted tubules and connecting tubules: effects of vasopressin and vitamin D3 on Ca2+ signaling.
Article Snippet: Hofmeister MV, Fenton RA, Praetorius J. Fluorescence isolation of mouse late distal convoluted tubules and connecting tubules: effects of vasopressin and vitamin D3 on Ca signaling.. Am J Physiol Renal Physiol 296: F194–F203, 2009.. First published November 5, 2008; doi:10.1152/ajprenal.90495.2008.—The renal late distal convoluted tubules and connecting tubules are sites for the fine regulation of Na and Ca reabsorption.

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